1-Deazaadenosine: synthesis and activity of base-modified hammerhead ribozymes
نویسندگان
چکیده
منابع مشابه
Minimal Hammerhead Ribozymes with Uncompromised Catalytic Activity.
We report here that a single additional trans-Hoogsteen base-pairing interaction in the minimal hammerhead ribozyme transforms an RNA sequence possessing typically modest catalytic activity into one possessing greatly enhanced catalytic activity that is instead typical of full-length natural hammerhead RNAs that have additional extensive tertiary contact interactions. Formation of this addition...
متن کاملNew strategy for the synthesis of chemically modified RNA constructs exemplified by hairpin and hammerhead ribozymes.
The CuAAC reaction (click chemistry) has been used in conjunction with solid-phase synthesis to produce catalytically active hairpin ribozymes around 100 nucleotides in length. Cross-strand ligation through neighboring nucleobases was successful in covalently linking presynthesized RNA strands with high efficiency (trans-ligation). In an alternative strategy, intrastrand click ligation was empl...
متن کاملRedesigned and chemically-modified hammerhead ribozymes with improved activity and serum stability
BACKGROUND: Hammerhead ribozymes are RNA-based molecules which bind and cleave other RNAs specifically. As such they have potential as laboratory reagents, diagnostics and therapeutics. Despite having been extensively studied for 15 years or so, their wide application is hampered by their instability in biological media, and by the poor translation of cleavage studies on short substrates to lon...
متن کاملIntronic hammerhead ribozymes in mRNA biogenesis
Small self-cleaving ribozymes are a group of natural RNAs that are capable of catalyzing their own and sequence-specific endonucleolytic cleavage. One of the most studied members is the hammerhead ribozyme (HHR), a catalytic RNA originally discovered in subviral plant pathogens but recently shown to reside in a myriad of genomes along the tree of life. In eukaryotes, most of the genomic HHRs se...
متن کاملFast and simple purification of chemically modified hammerhead ribozymes using a lipophilic capture tag.
A new type of 5'-lipophilic capture tag is described, enabling the facile reverse phase HPLC purification of chemically modified hammerhead ribozymes (oligozymes) whilst still carrying the 2'-O-tert.-butyldimethylsilyl protection of the essential riboses. In its most convenient form, the capture tag consists of a simple diol, such as hexan-1,6-diol, which at one end is attached via a silyl resi...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1998
ISSN: 1362-4962
DOI: 10.1093/nar/26.4.1010